Proposal of a non-invasive pelvic imobilization model: study in rats.

Abstract

The pelvic waist is a structure that holds great importance for locomotion. Interventions requiring pelvic immobilization deserve attention by the resulting functional loss. Thus, the study aimed to present a pelvic immobilization device for rats and evaluate the chemo-metabolic alterations on the pelvic musculature in question. Eighteen rats were divided into three groups (n = 6): control (C), pelvic immobilization for 7 days (IP) and remobilized for 7 days after 7 days of device use (RP7). Then analysis chemo-metabolic in pelvic muscles involved were done. The data were processed with the Kolmogorov-Smirnov test followed by ANOVA and Tukey test (p <0.05 for all tests). The glycogen reserves reduced on average 68% in the IP group and 50% in the RP7 group. The glucose decay constant (KITT) had a delay of 10% in the IP group. The ratio of total protein / DNA showed that there was a reduction of 28 and 24% in the gluteo maximus and 32 and 16% in the iliopsoas from the IP and RP7 groups, respectively. It found an increase of cytokines (IL-6 and 10) in the IP and RP7 groups, and only the IP group showed an increased of TNF-α. So the model was effective in inducing the disuse of the muscles in question.